\u8133\u79d1\u5b66\u8f9e\u5178\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc<\/a>\u3084\u907a\u4f1d\u5b50\u5c0e\u5165<\/a>\u306e\u9805\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n \u4f7f\u7528\u91cf\u3054\u3068\u306b\u5206\u6ce8\u3057\u3066\u3001\uff0d80\u2103\u3067\u306e\u4fdd\u5b58\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081<\/strong>\u3057\u307e\u3059\u3002\u305f\u3060\u3057\u3001AAV\u30d9\u30af\u30bf\u30fc\u306f\u6bd4\u8f03\u7684\u7269\u7406\u7684\u306b\u5b89\u5b9a\u3067\u3042\u308a\u3001\u5c11\u306a\u304f\u3068\u3082\u5e73\u4e95\u30b0\u30eb\u30fc\u30d7\u3067\u4f5c\u6210\u3057\u305fAAV2\/9\u3084AAV-PHP.eB\u306b\u95a2\u3057\u3066\u306f\u30014\u2103\u3067\u6570\u9031\u9593\u304b\u3089\u6570\u30f6\u6708\u5b89\u5b9a\u3067\u3042\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u306a\u304c\u3089\u3001\u9577\u671f\u9593\u306e\u51b7\u8535\u5eab\u3067\u306e\u4fdd\u5b58\u306e\u5834\u5408\u3001\u611f\u67d3\u80fd\u304c\u5f90\u3005\u306b\u843d\u3061\u3066\u3044\u304f\u53ef\u80fd\u6027\u306f\u5426\u5b9a\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3001\uff0d80\u2103\u3067\u306e\u4fdd\u5b58\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>\n \u7cbe\u88fd\u6e08\u307f\u304b\u3064D-PBS(-)\u306b\u7f6e\u63db\u6e08\u307f\u3067\u3059\u306e\u3067\u3001\u4efb\u610f\u306e\u6fc3\u5ea6(vg\/ml)\u306bPBS\u7b49\u3067\u5e0c\u91c8\u5f8c\u3001\u305d\u306e\u307e\u307e in vitro<\/em> \u304a\u3088\u3073 in vivo<\/em> \u5b9f\u9a13\u306b\u304a\u4f7f\u3044\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n AAV\u30d9\u30af\u30bf\u30fc\u306f\u30a8\u30f3\u30d9\u30ed\u30fc\u30d7\u3092\u6301\u305f\u306a\u3044\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3067\u3042\u308b\u305f\u3081\u3001\u30a2\u30eb\u30b3\u30fc\u30eb\uff0870\uff05\u30a8\u30bf\u30ce\u30fc\u30eb\u7b49\uff09\u3067\u306f\u4e0d\u6d3b\u5316\u3067\u304d\u307e\u305b\u3093<\/span><\/strong>\u3002\u5fc5\u305a\u3001\u6b21\u4e9c\u5869\u7d20\u9178\u6eb6\u6db2\uff08\u30cf\u30a4\u30bf\u30fc\u7b49\uff09\u306b\u3088\u308b\u51e6\u7406\u307e\u305f\u306f\u30aa\u30fc\u30c8\u30af\u30ec\u30fc\u30d6\u51e6\u7406\u3092\u884c\u3063\u3066\u304b\u3089\u5ec3\u68c4\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n \u7fa4\u99ac\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7cfb\u7814\u7a76\u79d1\u8133\u795e\u7d4c\u518d\u751f\u533b\u5b66\u5206\u91ce\uff08\u5e73\u4e95\u7814\uff09\u3067\u306f\u3001AAV\u30d9\u30af\u30bf\u30fc\u3092\u5229\u7528\u3057\u305f\u6570\u591a\u304f\u306e\u6587\u732e\u3092\u767a\u8868\u3057\u3066\u3044\u307e\u3059\u3002\u4e00\u4f8b\u3068\u3057\u3066\u3001\u4ee5\u4e0b\u306e\u6587\u732e1-3<\/sup>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n 1. Huda et al., (2014) Distinct transduction profiles in the CNS via three injection routes of AAV9 and the application to generation of a neurodegenerative mouse model. \u30de\u30a6\u30b9\u306e\u8840\u6db2\u8133\u95a2\u9580\uff08BBB\uff09\u3092\u9ad8\u52b9\u7387\u306b\u900f\u904e\u3067\u304d\u308b\u6027\u8cea\u3092\u6301\u3064\u305f\u3081\u3001\u9759\u8108\u6295\u4e0e\u306b\u3088\u308a\u8133\u5168\u57df\u306b\u907a\u4f1d\u5b50\u5c0e\u5165\u304c\u53ef\u80fd\u306aAAV\u30d9\u30af\u30bf\u30fc\u3067\u3042\u308bPHP.B4<\/sup>\u306e\u6539\u826f\u72485<\/sup>\u3067\u3059\u3002\u8a73\u3057\u304f\u306f\u4ee5\u4e0b\u306e\u8ad6\u6587\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001BALB\/c\u3067\u306fBBB\u3092\u900f\u904e\u3067\u304d\u306a\u30446<\/sup>\u305f\u3081\u3001\u4f7f\u7528\u3059\u308b\u30de\u30a6\u30b9\u306e\u7cfb\u7d71\u306b\u304a\u6c17\u3092\u4ed8\u3051\u4e0b\u3055\u3044\u3002\u3055\u3089\u306b\u3001\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u306b\u304a\u3044\u3066\u306f\u3001PHP.B\u306eBBB\u900f\u904e\u52b9\u7387\u306fAAV2\/9\u3068\u540c\u7a0b\u5ea6\u3067\u3042\u308a\u3001\u307b\u3068\u3093\u3069\u8133\u5185\u306b\u79fb\u884c\u3057\u307e\u305b\u30937<\/sup>\u3002<\/p>\n 4\uff0eDeverman et al., (2016) Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. \u57fa\u672c\u7684\u306b\u540c\u3058\u3082\u306e\u3068\u8003\u3048\u3066\u69cb\u3044\u307e\u305b\u3093\u3002AAV\u30d9\u30af\u30bf\u30fc\u306e\u8840\u6e05\u578b\u3092\u8868\u8a18\u3059\u308b\u5834\u5408\u3001\u6163\u4f8b\u7684\u306b AAV?\/?\u3068\u8a18\u8f09\u3057\u307e\u3059\u3002\u6700\u521d\u306e\u300c?\u300d\u306fReplicase\u306e\u8840\u6e05\u578b\u30012\u756a\u76ee\u306e\u300c?\u300d\u306bCapsid\u306e\u8840\u6e05\u578b\u3092\u8a18\u8f09\u3057\u307e\u3059\u3002\u5e73\u4e95\u30b0\u30eb\u30fc\u30d7\u3067\u4f5c\u6210\u3059\u308bAAV9\u306f\u3001AAV2\u7531\u6765\u306eReplicase\u3068\u3001AAV9\u7531\u6765\u306eCapsid\u3092\u542b\u3080\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u7528\u3044\u3066\u3044\u308b\u305f\u3081\u3001AAV2\/9\u3068\u8868\u8a18\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u7814\u7a76\u306b\u7528\u3044\u3089\u308c\u308b\u591a\u304f\u306eAAV\u7528\u767a\u73fe\u30d7\u30e9\u30b9\u30df\u30c9\uff08pAAV-xxx\uff09\u306f\u3001AAV2\u7531\u6765\u306eITR\u3092\u6301\u3063\u3066\u3044\u308b\u305f\u3081\u3001AAV2\u7531\u6765\u306eReplicase\u3092\u7528\u3044\u3066\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u52b9\u7387\u7684\u306bAAV\u7c92\u5b50\u3092\u7523\u751f\u3067\u304d\u307e\u3059\u3002<\/p>\n \u7fa4\u99ac\u5927\u5b66\u3067\u4f5c\u88fd\u3059\u308bAAV\u30d9\u30af\u30bf\u30fc\u306f\u3001\u57f9\u5730\u4e2d\u306b\u653e\u51fa\u3055\u305b\u305fAAV\u30d9\u30af\u30bf\u30fc\u306e\u307f\u3092\u56de\u53ce\u3057\u3001iodixanol\u3092\u7528\u3044\u305f\u8d85\u9060\u5fc3\u6cd5\u3067\u4f5c\u88fd\u3057\u3066\u3044\u307e\u3059\u3002\u8a73\u3057\u3044\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306f\u4ee5\u4e0b\u306e\u6587\u732e\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\u3002 Q1 \u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u306b\u3064\u3044\u3066\u6559\u3048\u3066\u304f\u3060\u3055\u3044 \u8133\u79d1\u5b66\u8f9e\u5178\u306e\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3084\u907a\u4f1d\u5b50\u5c0e\u5165\u306e\u9805\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002 Q2 AAV\u30d9\u30af\u30bf\u30fc\u306e\u4fdd\u5b58\u65b9\u6cd5\u306f\uff1f \u4f7f\u7528\u91cf\u3054\u3068\u306b\u5206\u6ce8\u3057\u3066\u3001\uff0d80\u2103\u3067\u306e\u4fdd\u5b58\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u305f\u3060\u3057\u3001AA […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":19,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1056","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/1056","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1056"}],"version-history":[{"count":26,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/1056\/revisions"}],"predecessor-version":[{"id":2400,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=\/wp\/v2\/pages\/1056\/revisions\/2400"}],"wp:attachment":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1056"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}Q2 AAV\u30d9\u30af\u30bf\u30fc\u306e\u4fdd\u5b58\u65b9\u6cd5\u306f\uff1f<\/h4>\n
Q3 \u3069\u306e\u3088\u3046\u306b\u30a6\u30a4\u30eb\u30b9\u6eb6\u6db2\u3092\u4f7f\u7528\u3057\u305f\u3089\u3044\u3044\u3067\u3057\u3087\u3046\u304b\uff1f<\/h4>\n
Q4 AAV\u30d9\u30af\u30bf\u30fc\u306e\u4e0d\u6d3b\u5316\u51e6\u7406\u306f\u3069\u306e\u3088\u3046\u306b\u884c\u3048\u3070\u3044\u3044\u3067\u3057\u3087\u3046\u304b\uff1f<\/h4>\n
Q5 AAV\u30d9\u30af\u30bf\u30fc\u3092\u4f7f\u7528\u3057\u305f\u6587\u732e\u306f\u3042\u308a\u307e\u3059\u304b\uff1f<\/h4>\n
\nMolecular Therapy-Methods & Clinical Development,\u00a01:14032.
\nhttps:\/\/www.sciencedirect.com\/science\/article\/pii\/S2329050116300997?via%3Dihub<\/a>
\n2.\u00a0Matsuzaki et al., (2016) Transduction Profile of the Marmoset Central Nervous System Using Adeno-Associated Virus Serotype 9 Vectors.
\nMolecular Neurobiology, 54(3):1745-1758.
\nhttps:\/\/link.springer.com\/article\/10.1007%2Fs12035-016-9777-6<\/a>
\n3.\u00a0Shinohara et al., (2018)\u00a0Effects of Neutralizing Antibody Production on AAV-PHP.B-Mediated Transduction of the Mouse Central Nervous System.
\nMol Neurobiol. 2018 Oct 5.
\nhttps:\/\/link.springer.com\/article\/10.1007%2Fs12035-018-1366-4<\/a>
\n4\uff0eMatsuzaki et al., (2019) Neurotropic Properties of AAV-PHP.B Are Shared among Diverse Inbred Strains of Mice.
\nMol Ther. 2019 Apr 10;27(4):700-704. Epub 2019 Feb 28.
\nhttps:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30842039<\/a>
\n5. Yasui et al., (2020) Global knockdown of retinoid-related orphan receptor \u03b1 in mature Purkinje cells reveals aberrant cerebellar phenotypes of spinocerebellar ataxia.
\nNeuroscience. 2020 Apr. doi: 10.1016\/j.neuroscience.2020.04.004.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/32278059\/<\/a>
\n6. Hoshino et al., (2021) GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter.
\nMol Brain. 2021 Feb 15;14(1):33.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/33588899\/<\/a>
\n7. Radhiyanti et al., (2021) Comparative study of neuron-specific promoters in mouse brain transduced by intravenously administered AAV-PHP.eB.<\/a>
\nNeurosci Lett. 2021 Jun 21;756:135956.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/33989730\/<\/a><\/p>\nQ6 AAV-PHP.eB\u3068\u306f\uff1f<\/h4>\n
\nNat Biotech, 34 (2):204-209.
\nhttps:\/\/www.nature.com\/articles\/nbt.3440<\/a>
\n5.\u00a0Chan et al., (2017) Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems.
\nNat Neurosci, 20:1172-1179.
\nhttps:\/\/www.nature.com\/articles\/nn.4593<\/a>
\n6\uff0eHordeaux et al., (2018) The Neurotropic Properties of AAV-PHP.B Are Limited to C57BL\/6J Mice.
\nMolecular Therapy, 26(3):664-668.
\nhttps:\/\/doi.org\/10.1016\/j.ymthe.2018.01.018<\/span><\/a>
\n7\uff0eMatsuzaki et al., (2018)\u00a0Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency in the marmoset brain.
\nNeurosci Lett, 665:182-188.
\nhttps:\/\/www.sciencedirect.com\/science\/article\/pii\/S0304394017309588?via%3Dihub<\/a>
\n<\/span><\/p>\nQ7 AAV2\/9\u3068\u306fAAV9\u3068\u306f\u7570\u306a\u308b\u306e\u3067\u3059\u304b\uff1f<\/h4>\n
Q8 AAV\u30d9\u30af\u30bf\u30fc\u306e\u7cbe\u88fd\u65b9\u6cd5\u3092\u6559\u3048\u3066\u4e0b\u3055\u3044\u3002<\/h4>\n
\nKonno & Hirai (2020) Efficient whole brain transduction by systemic infusion of minimally purified AAV-PHP.eB.
\nJournal of Neuroscience Methods, 2020. 346: p. 108914.
\nhttps:\/\/pubmed.ncbi.nlm.nih.gov\/32810474\/<\/a>
\n\u672c\u8ad6\u6587\u306f\u3001\u7c21\u6613\u7cbe\u88fd\u6cd5\u306b\u95a2\u3059\u308b\u8ad6\u6587\u3067\u3059\u304c\u3001\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3068\u3057\u3066\u3001\u8d85\u9060\u5fc3\u3067\u306e\u7cbe\u88fd\u6cd5\u3082\u8f09\u305b\u3066\u3042\u308a\u307e\u3059\u3002\u5e73\u4e95\u30b0\u30eb\u30fc\u30d7\u304b\u3089\u914d\u5e03\u3057\u305fAAV\u30d9\u30af\u30bf\u30fc\u4f7f\u7528\u3057\u305f\u7d50\u679c\u3092\u8ad6\u6587\u5316\u3059\u308b\u5834\u5408\u3001\u4e0a\u8a18\u8ad6\u6587\u3092\u5f15\u7528\u3057\u3066\u4e0b\u3055\u3044\u3002
\n\u4f8b\u6587\uff1a AAV vectors were produced using the ultracentrifugation method described in a previous paper (Konno & Hirai).<\/p>\n","protected":false},"excerpt":{"rendered":"