{"id":325,"date":"2024-06-11T10:23:41","date_gmt":"2024-06-11T01:23:41","guid":{"rendered":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/?page_id=325"},"modified":"2025-08-04T16:10:50","modified_gmt":"2025-08-04T07:10:50","slug":"%e3%82%bb%e3%83%b3%e3%82%bf%e3%83%bc%e3%81%a7%e6%a7%8b%e7%af%89%e6%b8%88%e3%81%ae%e3%83%97%e3%83%a9%e3%82%b9%e3%83%9f%e3%83%89","status":"publish","type":"page","link":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/?page_id=325","title":{"rendered":"\u30bb\u30f3\u30bf\u30fc\u3067\u4f5c\u88fd\u53ef\u80fd\u306aAAV\u8840\u6e05\u578b\u304a\u3088\u3073\u69cb\u7bc9\u6e08\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30ea\u30b9\u30c8"},"content":{"rendered":"
\n
\n

\u4f5c\u88fd\u53ef\u80fd\u306a\u8840\u6e05\u578b\u306e\u30ea\u30b9\u30c8<\/h3>\n<\/div>\n
\n\n\n\n\n
\n

\u5f97\u610f\u3068\u3059\u308b\u8840\u6e05\u578b<\/strong><\/h2>\n<\/td>\n

\n

AAV2\/9, PHP.eB<\/h2>\n<\/td>\n<\/tr>\n

\n

\u4f5c\u88fd\u53ef\u80fd\u306a\u8840\u6e05\u578b<\/strong><\/h2>\n<\/td>\n

\n

AAV2\/1, AAV2\/5, AAV2\/6, AAV2\/7, AAV2\/8, AAV2\/rh10, AAV2-retro, PHP.S, PHP.N, AAV.CAP-B10, AAV-F, AAV2-BR1, AAV2-BR1N, 9P31<\/h2>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b\u305d\u306e\u4ed6\u306e\u8840\u6e05\u578b\u3092\u5e0c\u671b\u3059\u308b\u5834\u5408\u306f\u5225\u9014\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\u3002<\/p>\n<\/div>\n<\/div>\n

\n
\n

\u69cb\u7bc9\u6e08\u307f\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30ea\u30b9\u30c8<\/h3>\n<\/div>\n
\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
ID<\/strong><\/td>\n\u767a\u73fe\u30d9\u30af\u30bf\u30fc<\/strong><\/td>\n\u7279\u5fb4<\/strong><\/td>\n<\/tr>\n
GA-001<\/td>\npAAV\/CBh_GFP-WPRE-HBGpA<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bGFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-002<\/td>\npAAV\/CBh_mCherry-WPRE-HBGpA<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bmCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-003<\/td>\npAAV\/CBh_Cre WPRE SV40pA<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bCre\u767a\u73fe<\/td>\n<\/tr>\n
GA-004<\/td>\npAAV\/CBh_GFP-P2A-Cre WPRE SV40<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bGFP-P2A-Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-005<\/td>\npAAV\/CBh_mCerulean3-WPRE<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bmCerulean3\u767a\u73fe<\/td>\n<\/tr>\n
GA-006<\/td>\npAAV\/CBh_mTurqoise2-WPRE<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bmTurqoise2\u767a\u73fe<\/td>\n<\/tr>\n
GA-007<\/td>\npAAV\/CBh_hrGFP II-WPRE<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bhrGFP II\u767a\u73fe<\/td>\n<\/tr>\n
GA-008<\/td>\npAAV\/CBh_H2B-GFP WPRE<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bH2B-GFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-009<\/td>\npAAV\/CBh_H2B-mCherry WPRE<\/td>\n\u30e6\u30d3\u30ad\u30bf\u30b9\u306bH2B-mCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-010<\/td>\npAAV\/CBh_FLEX-GFP WPRE<\/td>\nCre\u4f9d\u5b58\u7684\u306bGFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-011<\/td>\npAAV\/CBh_FLEX-mCherry WPRE<\/td>\nCre\u4f9d\u5b58\u7684\u306bmCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-012<\/td>\npAAV\/rNSE(1800)_GFP-WPRE-SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684GFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-013<\/td>\npAAV\/rNSE(1200)_GFP-WPRE-SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684GFP\u767a\u73fe, 1800\u30681200\u306eVersion\u6709\uff08\u6d3b\u6027\u306f1.8k>1.2k\uff09<\/td>\n<\/tr>\n
GA-014<\/td>\npAAV\/rNSE(1200)_mCherry WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684mCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-015<\/td>\npAAV\/rNSE(1200)_H2B-mCherry WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684H2B-mCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-016<\/td>\npAAV\/rNSE(1200)_Cre WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-017<\/td>\npAAV\/rNSE(1200)_GFP-P2A-Cre WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684GFP-P2A-Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-018<\/td>\npAAV\/rNSE(1200)_tTA-Adv. WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684tTA-Advance(Tet-off)\u767a\u73fe<\/td>\n<\/tr>\n
GA-019<\/td>\npAAV\/SynImCMV_GFP-WPRE<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684GFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-020<\/td>\npAAV\/SynImCMV_mCherry WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684mCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-021<\/td>\npAAV\/SynImCMV_Cre WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-022<\/td>\npAAV\/mCaMKII(1.3kb)_GFP WPRE SV40pA<\/td>\n\u795e\u7d4c\u7d30\u80de\u7279\u7570\u7684GFP\u767a\u73fe
\n(\u203b1.3-kb \u306f\u8208\u596e\u6027\u30cb\u30e5\u30fc\u30ed\u30f3\u3078\u306e\u7279\u7570\u6027\u304c\u3042\u307e\u308a\u9ad8\u304f\u306a\u3044[\u7279\u306b\u5927\u8133\u76ae\u8cea\u306b\u304a\u3044\u3066]\u3002\u8208\u596e\u6027\u30cb\u30e5\u30fc\u30ed\u30f3\u3078\u306e\u7279\u7570\u6027\u3092\u5f97\u305f\u3044\u5834\u5408\u306f\u30010.4-kb[GA-42]\u306e\u4f7f\u7528\u3092\u63a8\u5968)<\/td>\n<\/tr>\n
GA-023<\/td>\npAAV\/mL7-6_GFP WPRE<\/td>\n\u30d7\u30eb\u30ad\u30f3\u30a8\u7d30\u80de\u7279\u7570\u7684GFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-024<\/td>\npAAV\/mL7-6_Cre WPRE SV40pA<\/td>\n\u30d7\u30eb\u30ad\u30f3\u30a8\u7d30\u80de\u7279\u7570\u7684Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-025<\/td>\npAAV\/mL7-6_GFP-P2A-Cre WPRE SV40pA<\/td>\n\u30d7\u30eb\u30ad\u30f3\u30a8\u7d30\u80de\u7279\u7570\u7684GFP-P2A-Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-026<\/td>\npAAV\/mL7-6_tTA-Adv. WPRE SV40pA<\/td>\n\u30d7\u30eb\u30ad\u30f3\u30a8\u7d30\u80de\u7279\u7570\u7684tTA-Advance(Tet-off)\u767a\u73fe<\/td>\n<\/tr>\n
GA-027<\/td>\npAAV\/mGfap(ABC1D)_GFP WPRE SV40pA<\/td>\n\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\u7279\u7570\u7684GFP\u767a\u73fe\uff08\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u3067\u4f7f\u7528\u306e\u5834\u5408\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u7248cjGfap(ABC1D)\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\uff09<\/td>\n<\/tr>\n
GA-028<\/td>\npAAV\/mGfap(ABC1D)_mCherry WPRE SV40pA<\/td>\n\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\u7279\u7570\u7684mCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-029<\/td>\npAAV\/mGfap(ABC1D)_H2B-mCherry WPRE SV40pA<\/td>\n\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\u7279\u7570\u7684H2B-mCherry\u767a\u73fe<\/td>\n<\/tr>\n
GA-030<\/td>\npAAV\/mGfap(ABC1D)_Cre WPRE SV40pA<\/td>\n\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\u7279\u7570\u7684Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-031<\/td>\npAAV\/mGfap(ABC1D)_GFP-P2A-Cre WPRE SV40pA<\/td>\n\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\u7279\u7570\u7684GFP-P2A-Cre\u767a\u73fe<\/td>\n<\/tr>\n
GA-032<\/td>\npAAV\/mGfap(ABC1D)_tTA-Adv. WPRE SV40pA<\/td>\n\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\u7279\u7570\u7684tTA-Advance(Tet-off)\u767a\u73fe<\/td>\n<\/tr>\n
GA-033<\/td>\npAAV\/TRE_GFP WPRE<\/td>\ntTA\u5b58\u5728\u4e0b\u3067GFP\u3092\u767a\u73fe<\/td>\n<\/tr>\n
GA-034<\/td>\npAAV\/TRE_Venus WPRE<\/td>\ntTA\u5b58\u5728\u4e0b\u3067Venus\u3092\u767a\u73fe<\/td>\n<\/tr>\n
GA-035<\/td>\npAAV\/TRE_mCherry WPRE<\/td>\ntTA\u5b58\u5728\u4e0b\u3067mCherry\u3092\u767a\u73fe<\/td>\n<\/tr>\n
GA-036<\/td>\npAAV\/TRE_FLEX-GFP WPRE<\/td>\ntTA\u304a\u3088\u3073Cre\u5b58\u5728\u4e0b\u3067GFP\u3092\u767a\u73fe<\/td>\n<\/tr>\n
GA-037<\/td>\npAAV\/TRE_FLEX-mCherry WPRE<\/td>\ntTA\u304a\u3088\u3073Cre\u5b58\u5728\u4e0b\u3067mCherry\u3092\u767a\u73fe<\/td>\n<\/tr>\n
GA-038<\/td>\npAAV\/CBh_DIO-hM4D(Gi)-mCherry-SV40pA<\/td>\nCre\u4f9d\u5b58\u7684\u306b\u6291\u5236\u578bDREADD\u5206\u5b50\u767a\u73fe<\/td>\n<\/tr>\n
GA-039<\/td>\npAAV\/CBh_DIO-rM3D(Gs)-mCherry-SV40pA<\/td>\nCre\u4f9d\u5b58\u7684\u306b\u4fc3\u9032\u578bDREADD\u5206\u5b50\u767a\u73fe<\/td>\n<\/tr>\n
GA-040<\/td>\npAAV\/CBh_DIO-mCherry-SV40pA<\/td>\nCre\u4f9d\u5b58\u7684\u306bmCheery\u767a\u73fe (DREADD Control\u7528)<\/td>\n<\/tr>\n
GA-041<\/td>\npAAV\/mGAD65(delE1)_GFP WPRE SV40pA<\/td>\nGABA\u4f5c\u52d5\u6027\u30cb\u30e5\u30fc\u30ed\u30f3\u7279\u7570\u7684GFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-042<\/td>\npAAV\/mCaMKII(0.4kb)_GFP WPRE SV40pA<\/td>\n\u8208\u596e\u6027\u30cb\u30e5\u30fc\u30ed\u30f3\u7279\u7570\u7684GFP\u767a\u73fe<\/td>\n<\/tr>\n
GA-043<\/td>\npAAV\/mIba1_GFP WPRE miR-9.T miR-129-2-3p.T SV40pA<\/td>\nMicroglia\u7279\u7570\u7684GFP\u767a\u73fe
\n\uff08PHP.eB\u3067\u306f\u4f7f\u7528\u4e0d\u53ef\u3002\u8133\u3078\u306e\u76f4\u63a5\u6295\u4e0e\u306e\u307f\u3067\u4f7f\u7528\u53ef\u3002\uff09<\/td>\n<\/tr>\n
GA-044<\/td>\npAAV\/mIba1_GFP miR-9.T miR-129-2-3p.T WPRE miR-9.T miR-129-2-3p.T SV40pA<\/td>\nMicroglia\u7279\u7570\u7684GFP\u767a\u73fe[\u6539\u826f\u7248]<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u4f5c\u88fd\u53ef\u80fd\u306a\u8840\u6e05\u578b\u306e\u30ea\u30b9\u30c8 \u5f97\u610f\u3068\u3059\u308b\u8840\u6e05\u578b AAV2\/9, PHP.eB \u4f5c\u88fd\u53ef\u80fd\u306a\u8840\u6e05\u578b AAV2\/1, AAV2\/5, AAV2\/6, AAV2\/7, AAV2\/8, AAV2\/rh10, AAV2-retro, P […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-325","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=\/wp\/v2\/pages\/325","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=325"}],"version-history":[{"count":17,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=\/wp\/v2\/pages\/325\/revisions"}],"predecessor-version":[{"id":392,"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=\/wp\/v2\/pages\/325\/revisions\/392"}],"wp:attachment":[{"href":"https:\/\/synapse.dept.med.gunma-u.ac.jp\/vvcenter\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}