ウイルスベクター開発研究センター発足後の成果
2022年
- Inada K, Hagihara M, Tsujimoto K, Abe T, Konno A, Hirai H, Kiyonari H, Miyamichi K.
Plasticity of neural connections underlying oxytocin-mediated parental behaviors of male mice.
Neuron. 2022 Apr 12;S0896-6273(22)00304-X. doi: 10.1016/j.neuron.2022.03.033. Online ahead of print. - Ueda E, Ohta T, Konno A, Hirai H, Kurauchi Y, Katsuki H, Seki T.
D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation.
Cells. 2022 Apr 5;11(7):1230. doi: 10.3390/cells11071230. Online ahead of print. - Hirai H, Fukai Y, Konno A, Hosoi N.
Electrophysiological and Imaging Analysis of GFP-Tagged Protein Kinase C γ Translocation in Cerebellar Purkinje Cells.
Cerebellum. 2022 Feb 26. doi: 10.1007/s12311-022-01384-6. Online ahead of print. - Sakai A, Yasui T, Watanave M, Tatsumi R, Yamamoto Y, Takano W, Tani Y, Okamura I, Hirai H, Takeda S.
Development of novel potent ligands for GPR85, an orphan G protein-coupled receptor expressed in the brain.
Genes Cells. 2022 Feb 23. doi: 10.1111/gtc.12931. Online ahead of print. - Watanave M, Takahashi N, Hosoi N, Konno A, Yamamoto H, Yasui H, Kawachi M, Horii T, Matsuzaki Y, Hatada I, Hirai H.
Protein kinase Cγ in cerebellar Purkinje cells regulates Ca 2+-activated large-conductance K + channels and motor coordination
Proc Natl Acad Sci U S A. 2022 Feb 15;119(7):e2113336119. doi: 10.1073/pnas.2113336119. - Suzuki A, Kosugi S, Murayama E, Sasakawa E, Ohkawa N, Konno A, Hirai H, Inokuchi K.
A cortical cell ensemble in the posterior parietal cortex controls past experience-dependent memory updating.
Nat Commun. 2022 Jan 11;13(1):41. doi: 10.1038/s41467-021-27763-x - Ninomiya A, Mshaty A, Haijima A, Yajima H, Kokubo M, Khairinisa MA, Ariyani W, Fujiwara Y, Ishii S, Hosoi N, Hirai H, Amano I, Koibuchi N.
The neurotoxic effect of lactational PFOS exposure on cerebellar functional development in male mice.
Food Chem Toxicol. 2022 Jan;159:112751. doi: 10.1016/j.fct.2021.112751. Epub 2021 Dec 3.
2021年
- Cendelin J, Cvetanovic M, Gandelman M, Hirai H, Orr HT, Pulst SM, Strupp M, Tichanek F, Tuma J, Manto M.
Consensus Paper: Strengths and Weaknesses of Animal Models of Spinocerebellar Ataxias and Their Clinical Implications.
Cerebellum. 2021 Aug 10. doi: 10.1007/s12311-021-01311-1. Online ahead of print. - Kawakami R, Matsui M, Konno A, Kaneko R, Shrestha S, Shrestha S, Sunaga H, Hanaoka H, Goto S, Hosojima M, Kabasawa H, Obokata M, Koitabashi N, Matsui H, Sasaki T, Saito A, Yanagita M, Hirai H, Kurabayashi M, Iso T.
Urinary FABP1 is a biomarker for impaired proximal tubular protein reabsorption and is synergistically enhanced by concurrent liver injury.
J Pathol. 2021 Dec;255(4):362-373. doi: 10.1002/path.5775. Epub 2021 Sep 1. - Ohta T, Morikawa Y, Sato M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H, Seki T.
Therapeutic potential of d-cysteine against in vitro and in vivo models of spinocerebellar ataxia.
Exp Neurol. 2021 Sep;343:113791. doi: 10.1016/j.expneurol.2021.113791. Epub 2021 Jun 19. - Radhiyanti PT, Konno A, Matsuzaki Y, Hirai H.
Comparative study of neuron-specific promoters in mouse brain transduced by intravenously administered AAV-PHP.eB.
Neurosci Lett. 2021 Jun 21;756:135956. doi: 10.1016/j.neulet.2021.135956. Epub 2021 May 11. - Nagao S, Hirai H, Kano M, Yuzaki M.
Masao Ito-A Visionary Neuroscientist with a Passion for the Cerebellum.
Neuroscience. 2021 May 10;462:1-3. doi: 10.1016/j.neuroscience.2021.02.028. - Shuvaev AN, Belozor OS, Mozhei O, Yakovleva DA, Potapenko IV, Shuvaev AN, Smolnikova MV, Salmin VV, Salmina AB, Hirai H, Teschemacher AG, Kasparov S.
Chronic optogenetic stimulation of Bergman glia leads to dysfunction of EAAT1 and Purkinje cell death, mimicking the events caused by expression of pathogenic ataxin-1.
Neurobiol Dis. 2021 Jul;154:105340. doi: 10.1016/j.nbd.2021.105340. Epub 2021 Mar 19. - Sugimoto H, Horii T, Hirota JN, Sano Y, Shinoda Y, Konno A, Hirai H, Ishizaki Y, Hirase H, Hatada I, Furuichi T, Sadakata T.
The Ser19Stop single nucleotide polymorphism (SNP) of human PHYHIPL affects the cerebellum in mice.
Mol Brain. 2021 Mar 12;14(1):52. doi: 10.1186/s13041-021-00766-x. - Hoshino C, Konno A, Hosoi N, Kaneko R, Mukai R, Nakai J, Hirai H.
GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter.
Mol Brain. 2021 Feb 15;14(1):33. doi: 10.1186/s13041-021-00746-1. - Ohgami N, Iizuka A, Hirai H, Yajima I, Iida M, Shimada A, Tsuzuki T, Jijiwa M, Asai N, Takahashi M, Kato M.
Loss-of-function mutation of c-Ret causes cerebellar hypoplasia in mice with Hirschsprung disease and Down’s syndrome.
J Biol Chem. Jan-Jun 2021;296:100389. doi: 10.1016/j.jbc.2021.100389. Epub 2021 Feb 6.
2020年
- Inoue A, Kobayashi T, Hirai H, Kanaya N, Kohara K.
Protocol for BATTLE-1EX: A High-Resolution Imaging Method to Visualize Whole Synaptic Structures and their Components in the Nervous System.
J STAR Protoc. 2020 Nov 25;1(3):100166. doi: 10.1016/j.xpro.2020.100166. eCollection 2020 Dec 18. - Konno A, Hirai H.
Efficient whole brain transduction by systemic infusion of minimally purified AAV-PHP.eB.
J Neurosci Methods. 2020 Aug 15;346:108914. doi: 10.1016/j.jneumeth.2020.108914. - Sato M, Ohta T, Morikawa Y, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H, Seki T.
Ataxic phenotype and neurodegeneration are triggered by the impairment of chaperone-mediated autophagy in cerebellar neurons.
Neuropathol Appl Neurobiol. 2020 Jul 28. doi: 10.1111/nan.12649. - Sato M, Ueda E, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H, Seki T.
Glucocorticoids negatively regulates chaperone mediated autophagy and microautophagy.
Biochem Biophys Res Commun. 2020 Jul 12;528(1):199-205. doi: 10.1016/j.bbrc.2020.04.132. - Kohara K, Inoue A, Nakano Y, Hirai H, Kobayashi T, Maruyama M, Baba R, Kawashima C.
BATTLE: Genetically Engineered Strategies for Split-Tunable Allocation of Multiple Transgenes in the Nervous System.
iScience. 2020 Jun 26;23(6):101248. doi: 10.1016/j.isci.2020.101248 - Chao OY, Marron Fernandez de Velasco E, Pathak SS, Maitra S, Zhang H, Duvick L, Wickman K, Orr HT, Hirai H, Yang YM.
Targeting Inhibitory Cerebellar Circuitry to Alleviate Behavioral Deficits in a Mouse Model for Studying Idiopathic Autism.
Neuropsychopharmacology. 2020 Mar 16. doi: 10.1038/s41386-020-0656-5. - Yasui H, Matsuzaki Y, Konno A, Hirai H.
Global knockdown of retinoid-related orphan receptor α in mature Purkinje cells reveals aberrant cerebellar phenotypes of spinocerebellar ataxia.
Neuroscience. 2020 Apr. doi: 10.1016/j.neuroscience.2020.04.004. - Oe Y, Wang X, Patriarchi T, Konno A, Ozawa K, Yahagi K, Hirai H, Tsuboi T, Kitaguchi T, Tian L, McHugh TJ, Hirase H.
Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance.
Nature Communications. 2020 Jan 24;11(1):471. doi: 10.1038/s41467-020-14378-x.
2019年
- Matsumoto K, Mitani TT, Horiguchi SA, Kaneshiro J, Murakami TC, Mano T, Fujishima H, Konno A, Watanabe TM, Hirai H, Ueda HR.
Advanced CUBIC tissue clearing for whole-organ cell profiling.
Nature Protocols, 2019 Dec;14(12):3506-3537.
ウイルスベクター開発研究センター発足前の成果
-
アストロサイト特異的AAVベクター
Shinohara Y, Konno A, Takahashi N, Matsuzaki Y, Kishi S, Hirai H.
Viral Vector-Based Dissection of Marmoset GFAP Promoter in Mouse and Marmoset Brains.
PLoS One. 2016 Aug 29;11(8):e0162023. -
ニューロン特異的AAVベクター
Shinohara Y, Ohtani T, Konno A, Hirai H
Viral Vector-Based Evaluation of Regulatory Regions in the Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo.
Cerebellum. 2017 Dec;16(5-6):913-922. -
プルキンエ細胞特異的AAVベクター
Nitta K, Matsuzaki Y, Konno A, Hirai H
Minimal Purkinje Cell-Specific PCP2/L7 Promoter Virally Available for Rodents and Non-human Primates.
Mol Ther Methods Clin Dev. 2017 Jul 27;6:159-170. -
AAVカプシドに対する中和抗体産生
Shinohara Y, Konno A, Nitta K, Matsuzaki Y, Yasui H, Suwa J, Hiromura K, Hirai H.
Effects of Neutralizing Antibody Production on AAV-PHP.B-Mediated Transduction of the Mouse Central Nervous System.
Mol Neurobiol. 2019 Jun;56(6):4203-4214. -
マーモセット中枢神経系への遺伝子発現
Matsuzaki Y, Konno A, Mochizuki R, Shinohara Y, Nitta K, Okada Y, Hirai H
Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency in the marmoset brain.
Neurosci Lett. 2018 Feb 5;665:182-188.
Matsuzaki Y, Konno A, Mukai R, Honda F, Hirato M, Yoshimoto Y, Hirai H.
Transduction Profile of the Marmoset Central Nervous System Using Adeno-Associated Virus Serotype 9 Vectors.
Mol Neurobiol. 2017 Apr;54(3):1745-1758. -
血液脳関門透過型AAVベクター
Matsuzaki Y, Tanaka M, Hakoda S, Masuda T, Miyata R, Konno A, Hirai H
Neurotropic Properties of AAV-PHP.B Are Shared among Diverse Inbred Strains of Mice.
Mol Ther. 2019 Apr 10;27(4):700-704. Epub 2019 Feb 28.
