All Articles

今野 歩 講師

Ayumu Konno, PhD

E-mail: konnoa■gunma-u.ac.jp(■は@に変えてください)
ResearchMap: http://researchmap.jp/nowfielda/
Google Scholar: https://scholar.google.co.jp/citations?user=5F3MtVcAAAAJ&hl=ja
出身地: 宮城県仙台市
血液型: O型




この研究室は私がこれまで在籍したどの研究室よりも、研究に必要な機材が充実しており、とても研究をやりやすい環境です。また、分子生物学やタンパク質科学をやるチーム(通称: Molecular チーム)と電気生理をやるチーム(通称:電気生理チーム)で、ある程度の役割分担があるので、お互いに協力しながら大きな仕事を出来る可能性があります。また、ウイルスベクター(レンチウイルス・アデノ随伴ウイルス)などを初めとした、研究に必要な技術的ツールも充実しているので、アイディア次第で様々なことが出来る可能性がある研究室だと思います。



2003年3月 東北大学農学部応用生物化学科卒業
2008年3月 東北大学大学院生命科学研究科博士後期課程修了  取得学位: 博士(生命科学)


2006年4月 学術振興会特別研究員(DC2)
2008年4月 東北大学学際科学国際高等研究センター 博士研究員
2009年4月 東北大学生命科学研究科 グローバルCOE特任助教
2011年4月 群馬大学医学系研究科 助教
2016年8月~ 群馬大学医学系研究科 講師


    1. Matsumoto K, Mitani TT, Horiguchi SA, Kaneshiro J, Murakami TC, Mano T, Fujishima H, Konno A, Watanabe TM, Hirai H, Ueda HR (2019) Advanced CUBIC tissue clearing for whole-organ cell profiling. Nature Protocols. 2019 Nov 20. PMID: 31748753, doi: 10.1038/s41596-019-0240-9.
    2. Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, Sadakata T. (2019) Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments. J Neurosci. 2019 Jun 14. pii: 2002-18. PMID: 31201232 DOI: 10.1523/JNEUROSCI.2002-18.2019.
    3. Sato M, Seki T, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H. (2019) Rapamycin activates mammalian microautophagy. J Pharmacol Sci. 2019 May 24.pii: S1347-8613(19)34062-9. PMID: 31178328 DOI: 10.1016/j.jphs.2019.05.007.
    4. Matsuzaki Y, Tanaka M, Hakoda S, Masuda T, Miyata R, Konno A, Hirai H (2019) Neurotropic Properties of AAV-PHP.B Are Shared among Diverse Inbred Strains of Mice. Molecular Therapy. 2019 Feb 28. 27(4) 700-704. PMID: DOI: 10.1016/j.ymthe.2019.02.016
    5. Watanave M, Hoshino C, Konno A, Fukuzaki Y, Matsuzaki Y, Ishitani T, Hirai H (2019) Pharmacological enhancement of retinoid-related orphan receptor α function mitigates spinocerebellar ataxia type 3 pathology. Neurobiol Dis. 2018 Oct 18. pii: S0969-9961(18)30730-7. PMID: 30343032 DOI: 10.1016/j.nbd.2018.10.014.
    6. Seki T, Sato M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H. (2018) d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production. Mol Cell Neurosci. 2018 Oct 18. PMID: 30342995 DOI: 10.1016/j.mcn.2018.10.002.
    7. Shinohara Y†, Konno A†, Nitta K, Matsuzaki Y, Yasui H, Suwa J, Hiromura K, Hirai H. (2018) Effects of Neutralizing Antibody Production on AAV-PHP.B-Mediated Transduction of the Mouse Central Nervous System. Mol Neurobiol. 2018 Oct 5. †Contributed equally to this work
    8. Seki T, Sato M, Kibe Y, Ohta T, Oshima M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H. (2018) Lysosomal dysfunction and early glial activation are involved in the pathogenesis of spinocerebellar ataxia type 21 caused by mutant transmembrane protein 240. Neurobiol Dis. 2018 Sep 2. pii: S0969-9961(18)30519-9. DOI: 10.1007/s12035-018-1366-4.
    9. Ogawa Y, Kakumoto K, Yoshida T, Kuwako KI, Miyazaki T, Yamaguchi J, Konno A, Hata J, Uchiyama Y, Hirai H, Watanabe M, Darnell RB, Okano H, Okano HJ. (2018) Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci Rep. 2018 Feb 9;8(1):2722. doi: 10.1038/s41598-018-21130-5.
    10. Matsuzaki Y, Konno A, Mochizuki R, Shinohara Y, Nitta K, Okada Y, Hirai H (2018) Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency in the marmoset brain. Neuroscience Letters, 2017 Nov 24; 665:182-188. doi: 10.1016/j.neulet.2017.11.049.
    11. Takahashi N, Shuvaev AN, Konno A, Matsuzaki Y, Watanave M, Hirai H. (2017) Regulatory connection between the expression level of classical protein kinase C and pruning of climbing fibers from cerebellar Purkinje cells. Journal of Neurochemistry, doi: 10.1111/jnc.14239.
    12. Nitta K, Matsuzaki Y, Konno A, Hirai H. (2017) Minimal Purkinje cell-specific PCP2/L7 promoter virally available for rodents and non-human primates. Molecular Therapy – Methods & Clinical Development, Volume 6, 15 September 2017, Pages 159-170, doi: https://doi.org/10.1016/j.omtm.2017.07.006
    13. Harada K, Ito M, Wang X, Tanaka M, Wongso D, Konno A, Hirai H, Hirase H, Tsuboi T, Kitaguchi T. (2017) Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Scientific Reports. 2017 Aug 4; 7(1):7351. doi: 10.1038/s41598-017-07820-6
    14. Shinohara Y, Ohtani T, Konno A, Hirai H. (2017) Viral Vector-Based Evaluation of Regulatory Regions in the Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo. Cerebellum, 2017 May 15. doi: 10.1007/s12311-017-0866-5
    15. Tatsumi D, Nanatani K, Koike Y, Kamagata K, Takahashi S, Konno A, Furuta T, Sakurai M, Uozumi N (2017) Probing native metal ion association sites through quenching of fluorophores in the nucleotide binding domains of the ABC transporter MsbA. Biochemical Journal, 474(12), 1993-2007, doi: 10.1042/BCJ20161051
    16. Matsuzaki Y, Konno A, Mukai R, Honda F, Hirato M, Yoshimoto Y, Hirai H (2017) Transduction Profile of the Marmoset Central Nervous System Using Adeno-Associated Virus Serotype 9 Vectors. Molecular Neurobiology: 54(3):1745-1758. doi: 10.1007/s12035-016-9777-6
    17. Hattori T, Kaji M, Ishii H, Jureepon R, Takarada-Iemata M, Minh Ta H, Manh Le T, Konno A, Hirai H, Shiraishi Y, Ozaki N, Yamamoto Y, Okamoto H, Yokoyama S, Higashida H, Kitao Y, Hori O. (2017) CD38 positively regulates postnatal development of astrocytes cell-autonomously and oligodendrocytes non-cell-autonomously. Glia. 65(6):974-989. doi: 10.1002/glia.23139.
    18. Huda F, Fan Y, Suzuki M, Konno A, Matsuzaki Y, Takahashi N, Chan JK, Hirai H (2016) Fusion of Human Fetal Mesenchymal Stem Cells with “Degenerating” Cerebellar Neurons in Spinocerebellar Ataxia Type 1 Model Mice. PLoS ONE 11(11): e0164202.
    19. Arsenault J, Gholizadeh S, Niibori Y, Pacey LK, Halder SK, Koxhioni E, Konno A, Hirai H, Hampson DR (2016) FMRP Expression Levels in Mouse CNS Neurons Determine Behavioral Phenotype. Human Gene Therapy. 27(12):982-996.
    20. Shinohara Y†, Konno A†, Takahashi N, Matsuzaki Y, Kishi S, Hirai H (2016) Viral Vector-Based Dissection of Marmoset GFAP Promoter in Mouse and Marmoset Brains. PLoS ONE 11:e0162023. †Contributed equally to this work
    21. Sato M, Seki T, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H (2016) Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes to Cells. 21(8):861-73.
    22. Sawada Y, Konno A, Nagaoka J, Hirai H (2016) Inflammation-induced reversible switch of the neuron-specific enolase promoter from Purkinje neurons to Bergmann glia. Scientific Reports. 6, Article number: 27758
    23. Yanai T, Kurosawa A, Nikaido Y, Nakajima N, Saito T, Osada H, Konno A, Hirai H, Takeda S. (2016) Identification and molecular docking studies for novel inverse agonists of SREB, super conserved receptor expressed in brain. Genes to Cells. 2016 May 17. doi: 10.1111/gtc.12378.
    24. Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, Mikoshiba K, Itohara S, Nakai J, Iwai Y, Hirase H (2016) Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nature Communications 7.
    25. Iizuka A, Matsuzaki Y, Konno A, Hirai H. (2016) Plasticity of the developmentally arrested staggerer cerebellum in response to exogenous RORα. Brain Structure and Function, 221(6):2879-89. doi: 10.1007/s00429-015-1077-9.
    26. Huda F, Konno A, Matsuzaki Y, Goenawan H, Miyake K, Shimada T, Hirai H (2014) Distinct transduction profiles in the CNS via 3 injection routes of AAV9 and the application to generation of a neurodegenerative mouse model. Molecular Therapy – Methods and Clinical Development, 1, Article number: 14032.
    27. Saida H, Matsuzaki Y, Takayama K, Iizuka A, Konno A, Yanagi S, and Hirai H. (2014) One-year follow-up of gene expression by integrase-defective lentiviral vectors and their therapeutic potential in spinocerebellar ataxia model mice. Gene Therapy, 21:820-827.
    28. Konno A, Shuvaev AN, Miyake N, Miyake K, Iizuka A, Matsuura S, Huda F, Nakamura K, Yanagi S, Shimada T, Hirai H. (2014) Mutant Ataxin-3 with an Abnormally Expanded Polyglutamine Chain Disrupts Dendritic Development and Metabotropic Glutamate Receptor Signaling in Mouse Cerebellar Purkinje Cells. The Cerebellum, 13(1):29-41.
    29. Konno A, Honjo T, Uchida A, Ishizuka T, Yawo H (2011) Evaluation of a Sindbis virus vector displaying an immunoglobulin-binding domain: Antibody-dependent infection of neurons in living mice. Neuroscience Research 71:328-334.
    30. Konno A, Kitagawa A, Watanabe M, Ogawa T, Shirai T (2011) Tracing Protein Evolution through Ancestral Structures of Fish Galectin. Structure 19:711-721. Selected by Faculty of 1000
    31. Konno, A., Yonemaru, S., Kitagawa, A., Muramoto, K., Shirai, T. & Ogawa, T. (2010). Protein engineering of conger eel galectins by tracing of molecular evolution using probable ancestral mutants. BMC Evolutionary Biology 10, 43.
    32. Konno, A., Suzuki, Y., Ogawa, T. & Taniuchi, T. (2009). UV irradiation promotes the accumulation of triglyceride in Lipomyces lipofer. Biosci Biotechnol Biochem 73, 2474-2477.
    33. Konno, A., Ogawa, T., Shirai, T. & Muramoto, K. (2007). Reconstruction of a probable ancestral form of conger eel galectins revealed their rapid adaptive evolution process for specific carbohydrate recognition. Mol Biol Evol 24, 2504-2514.
    34. 小川 智久, 今野 歩, 村本 光二& 白井 剛 (2006). 加速進化によるタンパク質の耐熱化:進化工学による耐熱化機構の解明から. 生物物理 46, 201-208.
    35. Shionyu-Mitsuyama, C., Ito, Y., Konno, A., Miwa, Y., Ogawa, T., Muramoto, K. & Shirai, T. (2005). In vitro evolutionary thermostabilization of congerin II: a limited reproduction of natural protein evolution by artificial selection pressure. J Mol Biol 347, 385-397.


    1. 今野歩, 平井宏和 「ウイルスベクターの静脈投与によるマウス脳全域への遺伝子導入」実験医学 3月号. 2019; 37(4): 581-587
    2. Konno, A., Ogawa, T. & Shirai, T. (2010). Ancestral protein reconstruction as a new field in protein engineering. In Ribosomal Proteins and Protein Engineering (Ortendhal, V. & Salchow, H., eds.). Nova Science Publishers, NY.