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今野　歩　講師

Ayumu Konno, PhD

E-mail： konnoa■gunma-u.ac.jp（■は@に変えてください）
ResearchMap： http://researchmap.jp/nowfielda/
Google Scholar： https://scholar.google.co.jp/citations?user=5F3MtVcAAAAJ&hl=ja
出身地： 宮城県仙台市
血液型： O型

自己紹介

分野変更をしながら、いくつかの研究室を渡り歩いてきました。糖鎖生物学（分子進化工学）→生物工学（バイオマス研究）→神経科学。多様なバックグラウンドを活かしながら、研究を広げていきたいと思っています。

研究室について

この研究室は私がこれまで在籍したどの研究室よりも、研究に必要な機材が充実しており、とても研究をやりやすい環境です。また、分子生物学やタンパク質科学をやるチーム（通称： Molecular チーム）と電気生理をやるチーム（通称：電気生理チーム）で、ある程度の役割分担があるので、お互いに協力しながら大きな仕事を出来る可能性があります。また、ウイルスベクター（レンチウイルス・アデノ随伴ウイルス）などを初めとした、研究に必要な技術的ツールも充実しているので、アイディア次第で様々なことが出来る可能性がある研究室だと思います。

プロフィール

学歴

2003年3月 東北大学農学部応用生物化学科卒業
2008年3月 東北大学大学院生命科学研究科博士後期課程修了 　取得学位: 博士（生命科学）

職歴

2006年4月 学術振興会特別研究員（ＤＣ２）
2008年4月 東北大学学際科学国際高等研究センター 博士研究員
2009年4月 東北大学生命科学研究科 グローバルＣＯＥ特任助教
2011年4月 群馬大学医学系研究科 助教
2016年8月～ 群馬大学医学系研究科 講師
2019年10月〜 群馬大学 未来先端研究機構 ウイルスベクター開発研究センター 講師 (併任)
2021年11月〜2022年5月 オレゴン健康科学大学 Visiting Scientist

研究業績

  1. Morishima M, Matsumura S, Tohyama S,Nagashima T, Konno A, Hirai H and Watabe AM (2023) Excitatory subtypes of thelateral amygdala neurons are differentially involved in regulation of synaptic plasticity and excitation/inhibition balance in aversive learning in mice. Front. Cell. Neurosci. 17:1292822. doi: 10.3389/fncel.2023.1292822
  2. Ozawa K, Nagao M, Konno A, Iwai Y, Vittani M, Kusk P, Mishima T, Hirai H, Nedergaard M, Hirase H. (2023) Astrocytic GPCR-Induced Ca2+ Signaling Is Not Causally Related to Local Cerebral Blood Flow Changes. Int J Mol Sci. 2023 Sep 2;24(17):13590. PMID: 37686396, doi: 10.3390/ijms241713590
  3. Murase S, Sakitani N, Maekawa T, Yoshino D, Takano K, Konno A, Hirai H, Saito T, Tanaka S, Shinohara K, Kishi T, Yoshikawa Y, Sakai T, Ayaori M, Inanami H, Tomiyasu K, Takashima A, Ogata T, Tsuchimochi H, Sato S, Saito S, Yoshino K, Matsuura Y, Funamoto K, Ochi H, Shinohara M, Nagao M, Sawada Y. (2023) Interstitial-fluid shear stresses induced by vertically oscillating head motion lower blood pressure in hypertensive rats and humans. Nat Biomed Eng. 2023 Jul 6. PMID: 37414976, doi: 10.1038/s41551-023-01061-x
  4. Yaguchi K, Hagihara M, Konno A, Hirai H, Yukinaga H, Miyamichi K. (2023) Dynamic modulation of pulsatile activities of oxytocin neurons in lactating wild-type mice. PLoS One. 2023 May 10; 18(5):e0285589. PMID: 37163565, doi: 10.1371/journal.pone.0285589
  5. Kawabata H, Konno A, Matsuzaki Y, Hirai H (2023) A blood-brain barrier-penetrating AAV2 mutant created by a brain microvasculature endothelial cell-targeted AAV2 variant. Mol Ther Methods Clin Dev. 2023 Mar 2; 29:81-92. eCollection 2023 Jun 8. PMID: 36970652, doi: 10.1016/j.omtm.2023.02.016
  6. Jin M, Matsumoto S, Ayaki T, Yamakado H, Taguchi T, Togawa N, Konno A, Hirai H, Nakajima H, Komai S, Ishida R, Chiba S, Takahashi R, Takao T, Hirotsune S. (2022) DOPAnization of tyrosine in α-synuclein by tyrosine hydroxylase leads to the formation of oligomers. Nat Commun. 2022 Nov 12;13(1):6880. PMID: 36371400, doi: 10.1038/s41467-022-34555-4
  7. Okada Y, Hosoi N, Matsuzaki Y, Fukai Y, Hiraga A, Nakai J, Nitta K, Shinohara Y, Konno A, Hirai H. (2022) Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo. Commun Biol. 2022 Nov 11;5(1):1224. PMID: 36369525, doi: 10.1038/s42003-022-04200-3.
  8. Wang X, Delle C, Asiminas A, Akther S, Vittani M, Brøgger P, Kusk P, Vo CT, Radovanovic T, Konno A, Hirai H, Fukuda M, Weikop P, Goldman SA, Nedergaard M, Hirase H. (2022) Liver-secreted fluorescent blood plasma markers enable chronic imaging of the microcirculation. Cell Rep Methods. 2022 Oct 24; 2(10): 100302. Published online 2022 Sep 21. PMID: 36313804, doi: 10.1016/j.crmeth.2022.100302
  9. Tokuyama T, Uosaki H, Sugiura A, Nishitai G, Takeda K, Nagashima S, Shiiba I, Ito N, Amo T, Mohri S, Nishimura A, Nishida M, Konno A, Hirai H, Ishido S, Yoshizawa T, Shindo T, Takada S, Kinugawa S, Inatome R, Yanagi S (2022) Protective roles of MITOL against myocardial senescence and ischemic injury partly via Drp1 regulation, iScience. 2022 Jun 11;25(7):104582. PMID: 35789860, doi: 10.1016/j.isci.2022.104582
  10. Inada K, Hagihara M, Tsujimoto K, Abe T, Konno A, Hirai H, Kiyonari H, Miyamichi K. (2022) Plasticity of neural connections underlying oxytocin-mediated parental behaviors of male mice. Neuron. 2022 Jun 15;110(12):2009-2023.e5, PMID: 35443152, doi: 10.1016/j.neuron.2022.03.033
  11. Ueda E, Ohta T, Konno A, Hirai H, Kurauchi Y, Katsuki H, Seki T. (2022) D-Cysteine Activates Chaperone-Mediated Autophagy in Cerebellar Purkinje Cells via the Generation of Hydrogen Sulfide and Nrf2 Activation. Cells. 2022 Apr 5;11(7):1230. PMID: 35406792, doi: 10.3390/cells11071230
  12. Hirai H, Fukai Y, Konno A, Hosoi N. (2022) Electrophysiological and Imaging Analysis of GFP-Tagged Protein Kinase C γ Translocation in Cerebellar Purkinje Cells. Cerebellum. 2022 Feb 26. PMID: 35218526, doi: 10.1007/s12311-022-01384-6.
  13. Watanave M, Takahashi N, Hosoi N, Konno A, Yamamoto H, Yasui H, Kawachi M, Horii T, Matsuzaki Y, Hatada I, Hirai H. (2022) Protein kinase Cγ in cerebellar Purkinje cells regulates Ca2+-activated large-conductance K+ channels and motor coordination. Proc Natl Acad Sci U S A. 2022 Feb 15;119(7):e2113336119. PMID: 35145028 doi: 10.1073/pnas.2113336119
  14. Suzuki A, Kosugi S, Murayama E, Sasakawa E, Ohkawa N, Konno A, Hirai H, Inokuchi K. (2022) A cortical cell ensemble in the posterior parietal cortex controls past experience-dependent memory updating. Nature Communications 2022 Jan 11;13(1):41. PMID: 35017495, doi: 10.1038/s41467-021-27763-x
  15. Kawakami R, Matsui M, Konno A, Kaneko R, Shrestha S, Shrestha S, Sunaga H, Hanaoka H, Goto S, Hosojima M, Kabasawa H, Obokata M, Koitabashi N, Matsui H, Sasaki T, Saito A, Yanagita M, Hirai H, Kurabayashi M, Iso T. (2021) Urinary FABP1 is a biomarker for impaired proximal tubular protein reabsorption and is synergistically enhanced by concurrent liver injury. J Pathol. 2021 Aug 9. PMID: 34370295, doi: 10.1002/path.5775
  16. Ohta T, Morikawa Y, Sato M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H, Seki T. (2021) Therapeutic potential of d-cysteine against in vitro and in vivo models of spinocerebellar ataxia. Experimental Neurology, 2021 Jun 19;113791. PMID: 34157318, doi: 10.1016/j.expneurol.2021.113791
  17. Radhiyantia P, Konno A, Matsuzaki Y, Hirai H. (2021) Comparative study of neuron-specific promoters in mouse brain transduced by intravenously administered AAV-PHP.eB. Neuroscience Letter, 2021 May 11; 756:135956. PMID: 33989730, DOI: 10.1016/j.neulet.2021.135956
  18. Sugimoto H, Horii T, Hirota JN, Sano Y, Shinoda Y, Konno A, Hirai H, Ishizaki Y, Hirase H, Hatada I, Furuichi T, Sadakata T. (2021) The Ser19Stop single nucleotide polymorphism (SNP) of human PHYHIPL affects the cerebellum in mice. Molecular Brain, 2021 Mar 12;14(1):52. PMID: 33712038, doi: 10.1186/s13041-021-00766-x
  19. Hoshino C†, Konno A† (†Contributed equally to this work), Hosoi N, Kaneko R, Mukai R, Nakai J, Hirai H. (2021) GABAergic neuron-specific whole-brain transduction by AAV-PHP.B incorporated with a new GAD65 promoter. Molecular Brain, 2021 Feb 15;14(1):33. PMID: 33588899, doi: 10.1186/s13041-021-00746-1
  20. Konno A, Hirai H. (2020) Efficient whole brain transduction by systemic infusion of minimally purified AAV-PHP.eB. Journal of Neuroscience Methods, 2020 Aug 15;346:108914. PMID: 32810474, doi: 10.1016/j.jneumeth.2020.108914.
  21. Sato M, Ohta T, Morikawa Y, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H, Seki T. (2020) Ataxic phenotype and neurodegeneration are triggered by the impairment of chaperone-mediated autophagy in cerebellar neurons. Neuropathology and Applied Neurobiology, 2020 Jul 28. PMID: 32722888, doi: 10.1111/nan.12649.
  22. Sato M, Ueda E, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H, Seki T. (2020) Glucocorticoids negatively regulates chaperone mediated autophagy and microautophagy. Biochem Biophys Res Commun. 2020 May 30: S0006-291X(20)30872-X. doi: 10.1016/j.bbrc.2020.04.132.
  23. Yasui H, Matsuzaki Y, Konno A, Hirai H. (2020) Global Knockdown of Retinoid-Related Orphan Receptor α in Mature Purkinje Cells Reveals Aberrant Cerebellar Phenotypes of Spinocerebellar Ataxia. Neurosience, 2020 Apr 8:S0306-4522(20)30219-0. doi: 10.1016/j.neuroscience.2020.04.004.
  24. Oe Y, Wang X, Patriarchi T, Konno A, Ozawa K, Yahagi K, Hirai H, Tian L, McHugh TJ, Hirase H. (2020) Distinct temporal integration of noradrenaline signaling by astrocytic second messengers during vigilance. Nature Communications. 2020 Jan 24;11(1):471. PMID: 31980655, doi: 10.1038/s41467-020-14378-x.
  25. Matsumoto K, Mitani TT, Horiguchi SA, Kaneshiro J, Murakami TC, Mano T, Fujishima H, Konno A, Watanabe TM, Hirai H, Ueda HR (2019) Advanced CUBIC tissue clearing for whole-organ cell profiling. Nature Protocols. 2019 Nov 20. PMID: 31748753, doi: 10.1038/s41596-019-0240-9.
  26. Hosoi N, Shibasaki K, Hosono M, Konno A, Shinoda Y, Kiyonari H, Inoue K, Muramatsu SI, Ishizaki Y, Hirai H, Furuichi T, Sadakata T. (2019) Deletion of class II ARFs in mice causes tremor by the Nav1.6 loss in cerebellar Purkinje cell axon initial segments. J Neurosci. 2019 Jun 14. pii: 2002-18. PMID: 31201232 DOI: 10.1523/JNEUROSCI.2002-18.2019.
  27. Sato M, Seki T, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H. (2019) Rapamycin activates mammalian microautophagy. J Pharmacol Sci. 2019 May 24.pii: S1347-8613(19)34062-9. PMID: 31178328 DOI: 10.1016/j.jphs.2019.05.007.
  28. Matsuzaki Y, Tanaka M, Hakoda S, Masuda T, Miyata R, Konno A, Hirai H (2019) Neurotropic Properties of AAV-PHP.B Are Shared among Diverse Inbred Strains of Mice. Molecular Therapy. 2019 Feb 28. 27(4) 700-704. PMID: DOI: 10.1016/j.ymthe.2019.02.016
  29. Watanave M, Hoshino C, Konno A, Fukuzaki Y, Matsuzaki Y, Ishitani T, Hirai H (2019) Pharmacological enhancement of retinoid-related orphan receptor α function mitigates spinocerebellar ataxia type 3 pathology. Neurobiol Dis. 2018 Oct 18. pii: S0969-9961(18)30730-7. PMID: 30343032 DOI: 10.1016/j.nbd.2018.10.014.
  30. Seki T, Sato M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H. (2018) d-Cysteine promotes dendritic development in primary cultured cerebellar Purkinje cells via hydrogen sulfide production. Mol Cell Neurosci. 2018 Oct 18. PMID: 30342995 DOI: 10.1016/j.mcn.2018.10.002.
  31. Shinohara Y†, Konno A†, Nitta K, Matsuzaki Y, Yasui H, Suwa J, Hiromura K, Hirai H. (2018) Effects of Neutralizing Antibody Production on AAV-PHP.B-Mediated Transduction of the Mouse Central Nervous System. Mol Neurobiol. 2018 Oct 5. †Contributed equally to this work
  32. Seki T, Sato M, Kibe Y, Ohta T, Oshima M, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H. (2018) Lysosomal dysfunction and early glial activation are involved in the pathogenesis of spinocerebellar ataxia type 21 caused by mutant transmembrane protein 240. Neurobiol Dis. 2018 Sep 2. pii: S0969-9961(18)30519-9. DOI: 10.1007/s12035-018-1366-4.
  33. Ogawa Y, Kakumoto K, Yoshida T, Kuwako KI, Miyazaki T, Yamaguchi J, Konno A, Hata J, Uchiyama Y, Hirai H, Watanabe M, Darnell RB, Okano H, Okano HJ. (2018) Elavl3 is essential for the maintenance of Purkinje neuron axons. Sci Rep. 2018 Feb 9;8(1):2722. doi: 10.1038/s41598-018-21130-5.
  34. Matsuzaki Y, Konno A, Mochizuki R, Shinohara Y, Nitta K, Okada Y, Hirai H (2018) Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency in the marmoset brain. Neuroscience Letters, 2017 Nov 24; 665:182-188. doi: 10.1016/j.neulet.2017.11.049.
  35. Takahashi N, Shuvaev AN, Konno A, Matsuzaki Y, Watanave M, Hirai H. (2017) Regulatory connection between the expression level of classical protein kinase C and pruning of climbing fibers from cerebellar Purkinje cells. Journal of Neurochemistry, doi: 10.1111/jnc.14239.
  36. Nitta K, Matsuzaki Y, Konno A, Hirai H. (2017) Minimal Purkinje cell-specific PCP2/L7 promoter virally available for rodents and non-human primates. Molecular Therapy – Methods & Clinical Development, Volume 6, 15 September 2017, Pages 159-170, doi: https://doi.org/10.1016/j.omtm.2017.07.006
  37. Harada K, Ito M, Wang X, Tanaka M, Wongso D, Konno A, Hirai H, Hirase H, Tsuboi T, Kitaguchi T. (2017) Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging. Scientific Reports. 2017 Aug 4; 7(1):7351. doi: 10.1038/s41598-017-07820-6
  38. Shinohara Y, Ohtani T, Konno A, Hirai H. (2017) Viral Vector-Based Evaluation of Regulatory Regions in the Neuron-Specific Enolase (NSE) Promoter in Mouse Cerebellum In Vivo. Cerebellum, 2017 May 15. doi: 10.1007/s12311-017-0866-5
  39. Tatsumi D, Nanatani K, Koike Y, Kamagata K, Takahashi S, Konno A, Furuta T, Sakurai M, Uozumi N (2017) Probing native metal ion association sites through quenching of fluorophores in the nucleotide binding domains of the ABC transporter MsbA. Biochemical Journal, 474(12), 1993-2007, doi: 10.1042/BCJ20161051
  40. Matsuzaki Y, Konno A, Mukai R, Honda F, Hirato M, Yoshimoto Y, Hirai H (2017) Transduction Profile of the Marmoset Central Nervous System Using Adeno-Associated Virus Serotype 9 Vectors. Molecular Neurobiology: 54(3):1745-1758. doi: 10.1007/s12035-016-9777-6
  41. Hattori T, Kaji M, Ishii H, Jureepon R, Takarada-Iemata M, Minh Ta H, Manh Le T, Konno A, Hirai H, Shiraishi Y, Ozaki N, Yamamoto Y, Okamoto H, Yokoyama S, Higashida H, Kitao Y, Hori O. (2017) CD38 positively regulates postnatal development of astrocytes cell-autonomously and oligodendrocytes non-cell-autonomously. Glia. 65(6):974-989. doi: 10.1002/glia.23139.
  42. Huda F, Fan Y, Suzuki M, Konno A, Matsuzaki Y, Takahashi N, Chan JK, Hirai H (2016) Fusion of Human Fetal Mesenchymal Stem Cells with “Degenerating” Cerebellar Neurons in Spinocerebellar Ataxia Type 1 Model Mice. PLoS ONE 11(11): e0164202.
  43. Arsenault J, Gholizadeh S, Niibori Y, Pacey LK, Halder SK, Koxhioni E, Konno A, Hirai H, Hampson DR (2016) FMRP Expression Levels in Mouse CNS Neurons Determine Behavioral Phenotype. Human Gene Therapy. 27(12):982-996.
  44. Shinohara Y†, Konno A†, Takahashi N, Matsuzaki Y, Kishi S, Hirai H (2016) Viral Vector-Based Dissection of Marmoset GFAP Promoter in Mouse and Marmoset Brains. PLoS ONE 11:e0162023. †Contributed equally to this work
  45. Sato M, Seki T, Konno A, Hirai H, Kurauchi Y, Hisatsune A, Katsuki H (2016) Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes to Cells. 21(8):861-73.
  46. Sawada Y, Konno A, Nagaoka J, Hirai H (2016) Inflammation-induced reversible switch of the neuron-specific enolase promoter from Purkinje neurons to Bergmann glia. Scientific Reports. 6, Article number: 27758
  47. Yanai T, Kurosawa A, Nikaido Y, Nakajima N, Saito T, Osada H, Konno A, Hirai H, Takeda S. (2016) Identification and molecular docking studies for novel inverse agonists of SREB, super conserved receptor expressed in brain. Genes to Cells. 2016 May 17. doi: 10.1111/gtc.12378.
  48. Monai H, Ohkura M, Tanaka M, Oe Y, Konno A, Hirai H, Mikoshiba K, Itohara S, Nakai J, Iwai Y, Hirase H (2016) Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nature Communications 7.
  49. Iizuka A, Matsuzaki Y, Konno A, Hirai H. (2016) Plasticity of the developmentally arrested staggerer cerebellum in response to exogenous RORα. Brain Structure and Function, 221(6):2879-89. doi: 10.1007/s00429-015-1077-9.
  50. Huda F, Konno A, Matsuzaki Y, Goenawan H, Miyake K, Shimada T, Hirai H (2014) Distinct transduction profiles in the CNS via 3 injection routes of AAV9 and the application to generation of a neurodegenerative mouse model. Molecular Therapy – Methods and Clinical Development, 1, Article number: 14032.
  51. Saida H, Matsuzaki Y, Takayama K, Iizuka A, Konno A, Yanagi S, and Hirai H. (2014) One-year follow-up of gene expression by integrase-defective lentiviral vectors and their therapeutic potential in spinocerebellar ataxia model mice. Gene Therapy, 21:820-827.
  52. Konno A, Shuvaev AN, Miyake N, Miyake K, Iizuka A, Matsuura S, Huda F, Nakamura K, Yanagi S, Shimada T, Hirai H. (2014) Mutant Ataxin-3 with an Abnormally Expanded Polyglutamine Chain Disrupts Dendritic Development and Metabotropic Glutamate Receptor Signaling in Mouse Cerebellar Purkinje Cells. The Cerebellum, 13(1):29-41.
  53. Konno A, Honjo T, Uchida A, Ishizuka T, Yawo H (2011) Evaluation of a Sindbis virus vector displaying an immunoglobulin-binding domain: Antibody-dependent infection of neurons in living mice. Neuroscience Research 71:328-334.
  54. Konno A, Kitagawa A, Watanabe M, Ogawa T, Shirai T (2011) Tracing Protein Evolution through Ancestral Structures of Fish Galectin. Structure 19:711-721. Selected by Faculty of 1000
  55. Konno, A., Yonemaru, S., Kitagawa, A., Muramoto, K., Shirai, T. & Ogawa, T. (2010). Protein engineering of conger eel galectins by tracing of molecular evolution using probable ancestral mutants. BMC Evolutionary Biology 10, 43.
  56. Konno, A., Suzuki, Y., Ogawa, T. & Taniuchi, T. (2009). UV irradiation promotes the accumulation of triglyceride in Lipomyces lipofer. Biosci Biotechnol Biochem 73, 2474-2477.
  57. Konno, A., Ogawa, T., Shirai, T. & Muramoto, K. (2007). Reconstruction of a probable ancestral form of conger eel galectins revealed their rapid adaptive evolution process for specific carbohydrate recognition. Mol Biol Evol 24, 2504-2514.
  58. Shionyu-Mitsuyama, C., Ito, Y., Konno, A., Miwa, Y., Ogawa, T., Muramoto, K. & Shirai, T. (2005). In vitro evolutionary thermostabilization of congerin II: a limited reproduction of natural protein evolution by artificial selection pressure. J Mol Biol 347, 385-397.

著書

  1. 今野歩 「２章ウイルスベクターを用いた遺伝子導入プロトコール：６－静脈投与によるマウス脳全域への遺伝子導入」実験医学別冊 決定版 ウイルスベクターによる遺伝子導入実験ガイド, 2020年11月15日発行
  2. 今野歩 「１章各ウイルスベクターの特徴と作製の基本：２－AAVベクター」実験医学別冊 決定版 ウイルスベクターによる遺伝子導入実験ガイド, 2020年11月15日発行
  3. 今野歩, 平井宏和 「ウイルスベクターの静脈投与によるマウス脳全域への遺伝子導入」実験医学 ３月号 クローズアップ実験法. 2019; 37(4): 581-587平井宏和 「ウイルスベクターの静脈投与によるマウス脳全域への遺伝子導入」実験医学 ３月号. 2019; 37(4): 581-587
  4. Konno, A., Ogawa, T. & Shirai, T. (2010). Ancestral protein reconstruction as a new field in protein engineering. In Ribosomal Proteins and Protein Engineering (Ortendhal, V. & Salchow, H., eds.). Nova Science Publishers, NY.
  5. 小川 智久, 今野 歩, 村本 光二& 白井 剛 (2006). 加速進化によるタンパク質の耐熱化：進化工学による耐熱化機構の解明から. 生物物理 46, 201-208.

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